Thermo Fisher Scientific Phospho-Cyclin B1 (Ser147) Polyclonal Antibody
Cyclin B1의 Ser147 인산화 부위를 인식하는 Rabbit Polyclonal Antibody로, 세포주기 G2/M 전환 연구에 적합함. Western blot 및 IHC(Paraffin) 분석에 사용 가능. 액상 형태로 제공되며 -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147 (A-F-SP-D-V) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554961 |
Target Information
Cyclins bind to and regulate the activity of Cyclin-dependent protein kinases (CDKs). Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis and is essential for controlling the cell cycle at the G2/M transition. Cyclin B1 forms a complex with p34 (cdc2) to create the maturation-promoting factor (MPF).
Two alternative transcripts exist: one constitutively expressed and another cell cycle-regulated, predominantly during the G2/M phase. Cyclin B1 accumulates during G2 phase and undergoes APC-dependent degradation at mitosis end, which is crucial for cell cycle progression.
Cyclin B1 is overexpressed in several cancers, including breast, prostate, and non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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