Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Violet 650, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (ICRF44), Brilliant Violet 650, eBioscience™

Applications and Tested Dilution

• Flow Cytometry (Flow): 5 µL (0.125 µg)/test

Species Reactivity

• Baboon
• Chimpanzee
• Cynomolgus monkey
• Human
• Rhesus monkey

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV / Brilliant Violet / eFluor / FITC / NovaFluor / PE / Super Bright 시리즈
• Request custom conjugation

Product Specific Information

The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associates with integrin beta2 (CD18) and is expressed on monocytes, granulocytes, activated lymphocytes, and a subset of NK cells. It serves as a receptor for ICAM family members (CD54, CD102, CD50) and iC3b, playing a key role in cell-cell and cell-matrix interactions.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted; use at 5 µL (0.125 µg) per test
• Brilliant Violet™ 650 (BV650): Emits at 649 nm; use with violet (405 nm) laser
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize polymer interactions
• Light Sensitivity: Protect from light
• Fixation: Compatible with IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54); not compatible with methanol-based fixation
• Excitation / Emission / Laser: 407 nm / 649 nm / Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM) forms a heterodimer with CD18 (integrin beta-2) known as Mac-1 or CR3. It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 acts as a receptor for ICAMs, CD14, fibrinogen, and Factor X, mediating key adhesion and immune interactions. Expression varies by cell type and tissue, and is used as a microglial marker. Dysfunction is associated with systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures.

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