Thermo Fisher Scientific CEP135 Polyclonal Antibody

Applications

Western Blot (WB)

• Assay-dependent

Immunoprecipitation (IP)

• 5–10 µg/mg lysate

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with the cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle-dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors, and a high sensitivity to carcinogenic treatments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.