ELK Biotechnology Cryopyrin rabbit pAb

제품명

Cryopyrin rabbit pAb

대체 명칭

NLRP3; C1orf7; CIAS1; NALP3; PYPAF1; NACHT, LRR and PYD domains-containing protein 3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1CLR1.1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1

적용 분야 (Applications)

• WB
• IHC
• IF
• ELISA

권장 희석 배수 (Recommended Dilutions)

• Western Blot: 1/500 - 1/2000
• IHC-p: 1/100 - 1/300
• ELISA: 1/20000
  (Other applications not yet tested)

면역원 (Immunogen)

Synthesized peptide derived from the internal region of human NLRP3 (AA range: 511–560)

보관 및 안정성 (Storage)

• Host: Rabbit
• Storage condition: -20°C / 1 year

특성 (Properties)

세포 내 위치 (Cellular Localization)

Cytoplasm, cytosol, inflammasome, endoplasmic reticulum, secreted, nucleus.
In macrophages, under resting conditions, mainly located in the cytosol and on the endoplasmic reticulum. After stimulation with inducers of the NLRP3 inflammasome, mitochondria redistribute near the endoplasmic reticulum in the perinuclear region, resulting in colocalization of NLRP3 on the ER and PYCARD on mitochondria, allowing inflammasome activation. After pyroptosis induction, inflammasome specks are released extracellularly, promoting IL1B processing and engulfment by macrophages, which induces lysosomal damage and inflammasome activation (PubMed:24952504).

유전자 배경 (Background)

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. The protein interacts with apoptosis-associated speck-like protein PYCARD/ASC and is a component of the NALP3 inflammasome complex, which activates NF-κB signaling. It regulates inflammation, immune response, and apoptosis.
Mutations in this gene are linked to familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified.