Thermo Fisher Scientific BOB.1 Monoclonal Antibody (BOB1, 2421)
Human BOB1 단백질을 인식하는 Mouse IgG2b monoclonal antibody로, WB, IHC, Flow 등 다양한 응용에 적합. 고순도 Protein A/G 정제, 보존제 무첨가 PBS 용액 형태. B세포 항원 반응 및 germinal center 형성 관련 연구에 활용 가능.
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- 5450-MSM1-P1ABX
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BOB1, 2421 |
| Immunogen | Recombinant fragment (aa 148–255) of human BOB1 (POU2AF1) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It enhances OCT1-mediated promoter activity and, to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity and recognizes the POU domains of OCT1 and OCT2. Essential for B-cell antigen response and germinal center formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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