Thermo Fisher Scientific Kindlin 2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 380-550 of human FERMT2 (NP_0068231) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Kindlin 2,uniProtId:Q96AC1-1,ncbiNodeId:9606,antigenRange:380-550,antigenLength:680,antigenImageFileName:PA5-117148_Kindlin_2_Q96AC1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-117148_Kindlin_2_Q96AC1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.4 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901778

Product Specific Information

Positive Samples: A-549, HT-29, LO2, MCF7, HT-1080, Mouse liver

Immunogen sequence: KVFKPKKLTL KGYKQYWCTF KDTSISCYKS KEESSGTPAH QMNLRGCEVT PDVNISGQKF NIKLLIPVAE GMNEIWLRCD NEKQYAHWMA ACRLASKGKT MADSSYNLEV QNILSFLKMQ HLNPDPQLIP EQITTDITPE CLVSPRYLKK YKNKQITARI LEAHQNVAQM S

Target Information

Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.