Thermo Fisher Scientific ROBO1 Monoclonal Antibody (1F8)
Thermo Fisher Scientific의 ROBO1 Monoclonal Antibody (1F8)은 인간 ROBO1 단백질을 인식하는 Mouse IgG2a 항체입니다. Western blot과 ELISA에 적합하며, 액상 형태로 제공됩니다. PBS(pH 7.4)에 보존되며 방부제가 없습니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1–5 µg/mL
ELISA
- Tested Dilution: 0.1 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F8 |
| Immunogen | ROBO1 (NP_002932, 491–589 a.a.) partial recombinant protein with GST tag. MW of GST tag alone is 26 kDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
DGVLVSTQDS RIKQLENGVL QIRYAKLGDT GRYTCIASTP SGEATWSAYI EVQEFGVPVQ PPRPTDPNLI PSAPSKPEVT DVSRNTVTLS WQPNLNSGA
Target Information
Specialized cells at the midline, which separates the left and right halves of the CNS, have roles in directing growth cone behavior. In vertebrates, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents. Robo acts as a receptor for the repellent Slit and functions cell-autonomously. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels before reaching the midline and high levels after crossing. Robo1 and Robo2 are human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, and Robo2 to the zebrafish gene astray.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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