Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (5H10), Pacific Orange

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 19 publications 19 publications

Miscellaneous PubMed (Misc)

-

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5H10

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Pacific Orange™ Pacific Orange™ Pacific Orange™

View additional formats

• Alexa Fluor 488
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• APC-Alexa Fluor 750
• FITC
• Pacific Blue
• PE
• PE-Cyanine7
• PE-Texas Red
• PerCP
• Request custom conjugation

Excitation/Emission Max

400/551 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10376311

Product Specific Information

The 5H10 monoclonal antibody (mAb) recognizes the mouse CD8aplpha chain. 5H10 mAb has been used in other labs for flow cytometry and inhibition of T cell responses to IL-2. Every lot is tested by flow cytometry using freshly harvested mouse splenocytes.

If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Cells should be analyzed within 18 hours of staining for best results.

For Pacific Orange™ conjugates: excitation 405 nm/peak emission 551 nm. Matching isotype control is available: Rt IgG2b Pacific Orange™. Use a 575 or 585 nm band pass filter for optimal brightness from Pacific Orange™ dye.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.