Thermo Fisher Scientific cGAS Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 401-501 of human CGAS(NP_612450.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:cGAS,uniProtId:Q8N884-1,ncbiNodeId:9606,antigenRange:401-501,antigenLength:522,antigenImageFileName:PA5-121188_cGAS_Q8N884-1_Rabbit.svg,antigenImageFileNamePDP:PA5-121188_cGAS_Q8N884-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.14 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914760

Product Specific Information

Positive test controls include: U-87MG, Mouse testis, Mouse thymus, Rat lung. The target is usually found in the following locations: Cytoplasm, cytosol.

Immunogen sequence: EEKCCRKDCL KLMKYLLEQL KERFKDKKHL DKFSSYHVKT AFFHVCTQNP QDSQWDRKDL GLCFDNCVTY FLQCLRTEKL ENYFIPEFNL FSSNLIDKRS K

Target Information

cGAS (Cyclic GMP-AMP synthase) is a nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of 2, 5 phosphodiester linkage at the GpA step and the formation of 3, 5 phosphodiester linkage at the ApG step. This produces c[G(2,5)pA(35)p] responses. cGAS also binds cytosolic DNA directly, leading to the activation and synthesis of cGAMP. Then, a second messenger binds to and activates TMEM173/STING thereby triggering type-I interferon production. cGAS is also involved in innate immune sensorship of infection, detection of DNA from bacteria, response to cellular stress, DNA damage, and regulation of cellular senescence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.