Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), APC-eFluor 780, eBioscience

Thermo Fisher Scientific IgM Monoclonal Antibody (II/41), APC-eFluor 780, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• View 27 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant Violet 605
• Brilliant Violet 711
• eFluor 450
• eFluor 570
• eFluor 660
• FITC
• NovaFluor Red 710
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other mouse immunoglobulin classes (IgD, IgG, IgA). IgM is expressed intracellularly during early B cell development and on the surface of mature B cells. Fluorochrome-conjugated II/41 can be used to detect mouse IgM.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Validated by flow cytometric analysis of mouse bone marrow cells. Recommended usage ≤ 0.5 µg/test (100 µL final volume). Optimal titration required.

Fluorochrome Information:
APC-eFluor 780 emits at 780 nm and is excited with a 633 nm red laser. Ensure instrument compatibility.

Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.

Optical Properties:
Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is the first antibody produced during an immune response and is the largest immunoglobulin in serum. It consists of five heavy and five light chains, contributing to complement activation and antigen agglutination.
It is essential in innate immunity and widely used in research applications such as ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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