Thermo Fisher Scientific MUC1/CA15-3/EMA/CD227 (Epithelial Marker) Recombinant Mouse Monoclonal Antibody (r115D8)
Recombinant mouse monoclonal antibody recognizing epithelial marker MUC1/CA15-3/EMA/CD227. Validated for IHC (paraffin) with human samples. High specificity and purity (Protein A purified, 200 µg/mL). Suitable for cancer research applications.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Expression System | CHO cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | r115D8 |
| Immunogen | Recombinant full-length human MUC1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive Control: Colon, MCF-7 or MDA-231 cells. Human breast, ovarian, or endometrial carcinoma.
- Cellular Location: Cytoplasm, cell surface.
Target Information
MUC1 (Mucin 1, Episialin, MAM-6, CA 15-3, PEM, EMA) is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic lineages. It is a transmembrane glycoprotein with a large extracellular domain containing numerous O-linked glycans. MUC1 is highly polymorphic, with allelic variations leading to different repeat numbers (30–90) and molecular weights. It is abundantly expressed in over 90% of breast carcinomas and metastases.
Transgenic MUC1 associates with all four cebB receptors and localizes with erbB1 (EGFR) in lactating glands. The MUC1 gene has seven exons and multiple alternatively spliced variants. Overexpression, aberrant localization, and altered glycosylation are linked to carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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