Thermo Fisher Scientific CDK4 Monoclonal Antibody (DCS-35), Biotin

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA5-12981 targets Cdk4 in IF, IP, and WB applications and shows reactivity with Human, Mouse, Porcine, and Rat samples.
The MA5-12981 immunogen is purified recombinant cdk4 protein.

Target Information

CDK4/cyclin D3 serine/threonine kinase is a dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and plays a key role in the control of cell proliferation during the G1 phase of the cell cycle.
CDK4 forms a complex with D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). It mediates phosphorylation of the C-terminal region of Rb protein, leading to active transcriptional repression of the E2F complex. CDC37 and HSP90 preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of CDK4 and CDK2.
CDK6 and CDK4 both phosphorylate multiple residues in the Rb protein but show different residue selectivity in vitro — CDK6 phosphorylates Thr821, while CDK4 phosphorylates Thr826.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.