Thermo Fisher Scientific CDK4 Monoclonal Antibody (DCS-35), Biotin
CDK4 단백질을 표적하는 Biotin 결합 단일클론 항체로, Human, Mouse, Rat 등 다양한 종에서 반응합니다. Western blot, IP, IF 등 여러 응용에 적합하며, 고순도 Protein G 정제 및 안정적 PBS 저장 버퍼를 제공합니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- MA512981
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 11 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View publications |
| ELISA | – | View 1 publication |
| Immunoprecipitation (IP) | 2 µg/mL | View 5 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DCS-35 |
| Immunogen | Purified recombinant cdk4 protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10980320 |
Product Specific Information
MA5-12981 targets Cdk4 in IF, IP, and WB applications and shows reactivity with Human, Mouse, Porcine, and Rat samples.
The MA5-12981 immunogen is purified recombinant cdk4 protein.
Target Information
CDK4/cyclin D3 serine/threonine kinase is a dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and plays a key role in the control of cell proliferation during the G1 phase of the cell cycle.
CDK4 forms a complex with D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). It mediates phosphorylation of the C-terminal region of Rb protein, leading to active transcriptional repression of the E2F complex. CDC37 and HSP90 preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of CDK4 and CDK2.
CDK6 and CDK4 both phosphorylate multiple residues in the Rb protein but show different residue selectivity in vitro — CDK6 phosphorylates Thr821, while CDK4 phosphorylates Thr826.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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