Thermo Fisher Scientific CD20 Polyclonal Antibody
Rabbit 유래 CD20 폴리클로날 항체로 B세포 표면 단백질 검출에 사용. Western blot, IHC, ELISA, IP 등 다양한 응용 가능. 고순도 액상 형태로 제공되며 -20°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific CD20 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:10,000 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 1–5 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human CD20/MS4A1 Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Target Information
CD20 is a non-glycosylated surface phosphoprotein with a molecular weight of 33–37 kDa depending on phosphorylation. It is expressed on mature and most malignant B cells, a subset of T lymphocytes, and follicular dendritic cells. CD20 expression correlates with surface IgM and regulates calcium conductance, cell cycle progression, and B-cell proliferation.
It associates with lipid rafts and undergoes conformational changes upon extracellular triggering or BCR aggregation. After receptor ligation, BCR and CD20 colocalize and then dissociate before BCR endocytosis, while CD20 remains on the cell surface.
CD20 is a key therapeutic target for antibody-mediated B-cell depletion, as it remains membrane-bound and is not shed after monoclonal antibody treatment.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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