Thermo Fisher Scientific VSIV Protein L Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Vesicular stomatitis Indiana virus RNA-directed RNA polymerase L protein (598-784aa)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.865 mg/mL

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_2902318

Product Specific Information

This antibody has been tested in direct-ELISA

Target Information

RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.