Thermo Fisher Scientific VSIV Protein L Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:5,000

Product Specifications

Product Specific Information

This antibody has been tested in direct-ELISA.

Target Information

RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3′ leader promoter and transcribes all viral mRNAs with decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping occurs concomitantly with initiation of mRNA transcription. A GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain reaches a few nucleotides. Ribose 2′-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occurs by a stuttering mechanism at a slippery stop site present at the end of viral genes. After finishing transcription of one mRNA, the polymerase can resume transcription of the downstream gene.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.