Thermo Fisher Scientific HLA-DP/-DQ/-DR (MHC II) Monoclonal Antibody (CR3/43)
HLA-DP, DQ, DR(MHC II) 단백질을 인식하는 mouse monoclonal antibody로, 다양한 면역학적 분석(WB, IHC, ICC, Flow, ELISA)에 사용 가능. 인간 시료 반응성, Protein A/G 정제, 액상 형태로 제공되며 4°C 보관. 연구용 전용 제품.
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Thermo Fisher Scientific HLA-DP/-DQ/-DR (MHC II) Monoclonal Antibody (CR3/43)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg / 10^6 cells |
| ELISA | 2–4 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CR3/43 |
| Immunogen | Cells from human tonsil |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MHC class II molecules are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen-presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
Three loci—DR, DQ, and DP—encode the major expressed products of the human class II region. Human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells, playing a critical role in initiating immune responses.
HLA and MHC antibodies are essential tools in Immunopeptidomics, enabling identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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