Thermo Fisher Scientific CD253 (TRAIL) Monoclonal Antibody (N2B2), PE, eBioscience

Thermo Fisher Scientific CD253 (TRAIL) Monoclonal Antibody (N2B2), PE, eBioscience™

Applications

• Flow Cytometry

Tested Dilution: 0.125 µg/test
Publications: View 11 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Functional Grade
• Request custom conjugation

Product Specific Information

The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3^- NK1.1^+ NK cells after stimulation with IL-2 or IL-15.
N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse CD253-transfected cells. Recommended ≤ 0.125 µg/test (100 µL final volume). Cell number range: 10^5–10^8 cells/test. Antibody titration recommended for optimal performance.

Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

TRAIL is a cytokine belonging to the TNF ligand family. It preferentially induces apoptosis in transformed and tumor cells, but not in normal cells. TRAIL binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly TNFRSF11B/OPG.
Its activity may be modulated by decoy receptors that cannot induce apoptosis. Binding of TRAIL to its receptors activates MAPK8/JNK, caspase 8, and caspase 3.
TRAIL is a type II membrane protein expressed in various tissues and induces apoptosis and NF-κB activation similar to TNF and Fas ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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