Thermo Fisher Scientific eZKine CD8 Activation 1 Whole Blood Intracellular Cytokine Kit

Invitrogen™ eZKine™ CD8 Activation 1 Whole Blood Intracellular Cytokine Kit

This eZKine™ CD8 Activation 1 Whole Blood Intracellular Cytokine Kit is designed to rapidly identify cytokine-producing T lymphocytes of the CD8+ T cell lineage after stimulation of whole peripheral blood samples.

Stimulation of whole blood in the presence of Protein Transport Inhibitor Cocktail (cat. 00-4980, not included) is followed by red blood cell lysis and fixation in a single step and subsequent permeabilization. Samples are then ready to stain with the CD8 Activation 1 cocktail and concentration-matched Isotype Control cocktail.

Background

CD8+ T cells, or cytotoxic T lymphocytes, are immune cells responsible for the detection and clearance of virally or bacterially infected host cells. Upon recognition of antigen displayed on infected cells, activated CD8+ T cells upregulate early activation markers such as CD69 and FAS ligand, followed by the release of granules containing granzymes and perforin, as well as secretion of cytokines such as IFN gamma and TNF alpha.

Granzymes and perforin directly lyse infected target cells, while cytokines have paracrine effects including recruitment and activation of macrophages, lymphocytes, and PMNs. IFN gamma also upregulates MHC class I and II expression and promotes Th1 cell differentiation.

Specifications