Thermo Fisher Scientific NEK3 Polyclonal Antibody
Thermo Fisher Scientific의 NEK3 Polyclonal Antibody는 Human, Mouse, Rat 시료에 반응하는 Rabbit IgG 항체입니다. Western blot과 ELISA에 적합하며, 1.52 mg/mL 농도의 액상 형태로 제공됩니다. Affinity Chromatography로 정제되었으며, -20°C에서 보관합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 267–506 of human NEK3 (NP_689933.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.52 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855432 |
Product Specific Information
Immunogen sequence:
PEIIMEYGEE VLEEIKNSKH NTPRKKTNPS RIRIALGNEA STVQEEEQDR KGSHTDLESI NENLVESALR RVNREEKGNK SVHLRKASSP NLHRRQWEKN VPNTALTALE NASILTSSLT AEDDRGGSVI KYSKNTTRKQ WLKETPDTLL NILKNADLSL AFQTYTIYRP GSEGFLKGPL SEETEASDSV DGGHDSVILD PERLEPGLDE EDTDFEEEDD NPDWVSELKK RAGWQGLCDR
Target Information
NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis. NIMA-related mammalian proteins have since been identified as Nek1, Nek2, Nek3, and Nek4 (also designated STK2 or NRK2). High expression of Nek1 is seen in male and female germ cell lines of mouse. Nek2 is the closest known mammalian relative to NIMA. Like NIMA, Nek2 expression peaks at the G2 to M phase transition. Nek3 is a predominantly cytoplasmic enzyme detectable in all organs studied. Levels of Nek3 remain unchanged throughout the cell cycle but appear elevated in G0-arrested, quiescent fibroblasts. In developing testicular germ cells, differential expression patterns of Nek1, Nek2, and Nek4 indicate possible overlapping but distinct functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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