Thermo Fisher Scientific Phospho-STAT4 (Ser721) Polyclonal Antibody

Applications and Tested Dilution

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human STAT4 (Phospho-Ser721).

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage Buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage Conditions

-20°C

Shipping Conditions

Wet ice

RRID

AB_2662863

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Product Specific Information

Phospho-STAT4 (Ser721) Polyclonal Antibody detects endogenous levels of STAT4 only when phosphorylated at Ser721.

Target Information

STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 can be tyrosine phosphorylated by Jak1 or Jak2, forming homo- and heterodimers with related STAT family members. Tyrosine-phosphorylated STAT4 binds the IFN-gamma activated site (GAS), and serine phosphorylation is required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen, and testis.

STAT4 is activated by interleukin 12 (IL-12), which is necessary for IFN-gamma induction and Th1 response development, crucial for defense against intracellular pathogens. STAT4-deficient mice show impaired IL-12-mediated Th1 cell development and enhanced Th2 differentiation. In viral infection, IFN-α/β activation of STAT4 is required for IFN-γ production.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.