Thermo Fisher Scientific V5 Tag Recombinant Rabbit Monoclonal Antibody (BLRE03H)
V5 태그 단백질 검출용 토끼 단클론 항체로, Western blot, ICC/IF, IP에 최적화되어 있습니다. HEK293F 발현 시스템에서 생산된 재조합 항체이며, 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Immunoprecipitation (IP) | 20 µL/mg of lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLRE03H |
| Immunogen | Peptide representing the V5 Epitope tag (GKPIPNPLLGLDST) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 250 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE cell sections.
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or poorly immunogenic proteins when specific antibodies are not available.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5).
The V5 tag typically consists of 14 amino acids (GKPIPNPLLGLDST), though shorter sequences (IPNPLLGLD) are also used.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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