Thermo Fisher Scientific Rabbit anti-Human IgG (H+L) Secondary Antibody, HRP

Applications

Tested Dilution

Western Blot (WB)

1:5,000 - 1:25,000

Immunohistochemistry (IHC)

1:1,000-1:5,000

ELISA (ELISA)

1:150000-1:250000

Dot blot (DB)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

human IgG whole molecule

Conjugate

HRP HRP HRP

View additional formats

• [AP](/antibody/secondary/product-name/Human IgG (H--L)/conjugates/AP/host/Rabbit/)
• [Biotin ](/antibody/secondary/product-name/Human IgG (H--L)/conjugates/Biotin/host/Rabbit/)
• [FITC](/antibody/secondary/product-name/Human IgG (H--L)/conjugates/FITC/host/Rabbit/)
• [Texas Red](/antibody/secondary/product-name/Human IgG (H--L)/conjugates/Texas Red/host/Rabbit/)
• [TRITC](/antibody/secondary/product-name/Human IgG (H--L)/conjugates/TRITC/host/Rabbit/)
• Request custom conjugation

Form

Liquid

Concentration

2.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.01% gentamicin sulfate

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10984558

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.