Thermo Fisher Scientific MLH1 Monoclonal Antibody (4C9C7)
MLH1 단백질을 검출하는 Mouse monoclonal antibody. Western blot, IHC, ICC/IF, ELISA에 사용 가능. Human 및 비인간 영장류 시료에 반응. 85kDa의 MLH1 단백질을 인식하며, 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4C9C7 |
| Immunogen | Purified recombinant fragment of MLH1 (aa381–483) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10981391 |
Product Specific Information
MA5-15431 targets MLH1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and Non-human primate samples.
The immunogen is a purified recombinant fragment of MLH1 (aa381–483) expressed in E. coli.
MA5-15431 detects MLH1, which has a predicted molecular weight of approximately 85 kDa.
Target Information
MLH1 is a DNA mismatch repair protein essential for maintaining the integrity of genetic information.
Defects in mismatch repair pathways can lead to microsatellite instability (MSI), which is associated with carcinogenesis.
Mutations in mismatch repair genes, including MLH1, are linked to hereditary nonpolyposis colon cancer (HNPCC).
MLH1 functions with MSH2 and PMSH in the recognition and repair of mismatched nucleotides during DNA replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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