Thermo Fisher Scientific Phospho-ASK1 (Ser83) Polyclonal Antibody
인간 ASK1 Ser83 인산화 부위를 인식하는 Rabbit Polyclonal 항체로 Western blot과 ELISA에 적합. 고순도 친화 크로마토그래피 정제, 액상 형태로 제공되며 안정적인 PBS/glycerol buffer에 저장. 연구용으로 세포 신호전달 및 apoptosis 관련 연구에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
ELISA
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphorylated peptide around S83 of human MAP3K5 (NP_0059141) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.07 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901799 |
Product Specific Information
- Positive Samples: K-562
- Immunogen sequence: GSSVG
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase abundantly expressed in human heart and pancreas. It is activated in response to various extracellular stimuli, including cytokines, growth factors, and environmental stresses.
ASK1 (apoptosis signal-regulating kinase 1) activates two MAPKK subgroups, MKK4 and MKK6, which in turn activate JNK and p38 MAP kinases.
ASK1/MAPKKK5 is activated by TNFR and Fas through interactions with TRAF family members and the Fas-associated protein Daxx.
Overexpression of ASK1 induces apoptotic cell death, while a catalytically inactive form inhibits TNF-α-induced apoptosis.
ASK1 is expressed in various human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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