Thermo Fisher Scientific ATGL Monoclonal Antibody (494702)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per million cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

494702

Immunogen

E. coli-derived recombinant human PEDF R/PNPLA2 Val162-Thr332 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATGL,uniProtId:Q96AD5-1,ncbiNodeId:9606,antigenRange:162-332,antigenLength:504,antigenImageFileName:MA5-24202_ATGL_Q96AD5-1_House_mouse.svg,antigenImageFileNamePDP:MA5-24202_ATGL_Q96AD5-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2606183

Product Specific Information

In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse PEDF R is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.