Thermo Fisher Scientific VEGF Monoclonal Antibody (OTI2E11), TrueMAB

Applications and Tested Dilution

• Western Blot (WB): 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

VEGF (vascular endothelial growth factor) is a 45 kDa homodimeric, disulfide-linked glycoprotein that plays a critical role in angiogenesis, promoting tumor progression and metastasis. It enhances endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability.
The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptors (VEGFR-1/FLT1 and VEGFR-2/FLK1/KDR) are key regulators of angiogenic signaling, primarily expressed on endothelial cells.
VEGF signaling is essential for embryonic vascular development. A soluble form of FLT1 (sFLT1) acts as an inhibitor of angiogenesis by binding VEGF with high affinity. Elevated VEGF levels are associated with POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes), also known as Crow-Fukase syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.