Thermo Fisher Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit

Thermo Fisher Scientific Pierce™ Mouse Embryonic Fibroblast Isolation Kit

The Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos.

Features

• Optimized: Procedure and reagents optimized for viability, yield, purity, and ease of use
• High Yield: Provides a 2-fold increase in yield compared to do-it-yourself methods and other kits
• Functional: Cultured fibroblasts express appropriate biochemical markers

Includes

• Fibroblast Isolation Enzyme
• Hanks' Balanced Salt Solution (HBSS)
• DMEM for Primary Cell Isolation

Description

This kit is designed to obtain high viability and purity of embryonic fibroblasts using validated reagents and an optimized procedure.
It contains a tissue-specific dissociation enzyme and media formulated for primary cell culture, promoting the isolation and growth of functional fibroblasts.
MEF isolated and cultured with this kit can be cryopreserved for future use with minimal loss of viability.

Applications

• Generation of immortalized cell lines from transgenic mice
• Feeder layer for stem cells
• Immunohistochemistry (IHC) and immunofluorescence (IF) studies

Because it uses an optimized tissue-specific dissociation enzyme, this kit isolates fibroblasts with greater yield and cell viability than existing methods.
Isolated MEFs express fibroblast-specific protein markers, confirming their viability and metabolic integrity.
After isolation, mouse fibroblasts can be immortalized by continuous passage to establish unique cell lines and used as a cell culture model for studies such as gene regulation and stem cell research [1,2,3,4].

More Product Data

• An improved method for highly efficient isolation of primary mouse embryonic fibroblasts

Related Products

• Mouse Embryonic Fibroblast Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits

Specifications