Thermo Fisher Scientific DCP1A Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1–3 µg/mL

Product Specifications

Product Specific Information

• This antibody is predicted to react with mouse and rat based on sequence homology.
• Tested in Peptide ELISA: antibody detection limit dilution 1:16,000.

Target Information

Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4.
SMIF (Smad4-interacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration enhances the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through an EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF, as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-beta-induced nuclear translocation of SMIF.
The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of approximately 70 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.