Thermo Fisher Scientific U2AF1 Polyclonal Antibody
Thermo Fisher Scientific의 U2AF1 Polyclonal Antibody는 인간 및 마우스 반응성을 가진 고품질 항체로, WB, IHC, ICC, IP 등 다양한 응용에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 장기 보관 시 -20°C에서 안정적 유지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 23–227 of Human U2AF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.34 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545986 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji
- Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (94%), Chicken (98%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
U2AF1 gene belongs to the splicing factor SR family. The U2 auxiliary factor, composed of large and small subunits, is a non-snRNP protein essential for U2 snRNP binding to the pre-mRNA branch site. The small subunit encoded by this gene plays a critical role in RNA splicing by mediating interactions between the large subunit and enhancer-bound proteins. Alternative splicing variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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