Thermo Fisher Scientific PSME1 Recombinant Rabbit Monoclonal Antibody (JU60-33)
PSME1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, IP 등 다양한 실험에 적합합니다. HEK293 세포에서 발현되었으며, Protein A로 정제된 고순도 항체입니다. 인간 시료에 반응하며 -20°C에서 보관 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU60-33 |
| Immunogen | Recombinant protein within Human PSME1 aa 1–155 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_2848553 |
Product Specific Information
Positive Control: MCF-7, rat lung tissue lysate, HepG2, PC-3M, rat kidney tissue, human tonsil tissue, human liver cancer tissue, human prostate tissue, mouse small intestine tissue.
Target Information
Proteolytic degradation is essential for maintaining proper levels of short-lived and regulatory proteins involved in cellular metabolism, stress response, antigen presentation, receptor modulation, ion channel regulation, cell cycle control, transcription, and signaling. The ubiquitin-proteasome pathway degrades most proteins in the cytosol and nucleus, while others are processed via the vacuolar pathway involving endosomes, lysosomes, and the endoplasmic reticulum.
The 26S proteasome is an ATP-dependent, multisubunit (~31) molecular complex (~2.5 MDa) composed of a 20S proteolytic core and one or two 19S regulatory subunits. The 19S subunits recognize ubiquitinated proteins, unfolding and translocating them into the 20S lumen for degradation. Defects in this pathway are linked to genetic diseases such as cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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