ELK Biotechnology Ribosomal Protein L7 rabbit pAb
Ribosomal Protein L7에 특이적인 토끼 폴리클로날 항체로, WB, IHC, IF, ELISA에 사용 가능. 인체 유래 합성 펩타이드로 면역화되어 높은 특이성과 재현성을 제공. 세포질 및 핵 내 위치 단백질 검출에 적합.
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제품명
Ribosomal Protein L7 rabbit pAb
제품 정보
| 항목 | 내용 |
|---|---|
| Alternative Names | RPL7; 60S ribosomal protein L7 |
| Applications | WB; IHC; IF; ELISA |
| Recommended Dilutions | Western Blot: 1/500 - 1/2000 Immunohistochemistry: 1/100 - 1/300 ELISA: 1/10000 Other applications: Not yet tested |
| Immunogen | Synthesized peptide derived from human RPL7 (AA range: 199–248) |
| Host | Rabbit |
| Storage | -20°C / 1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band | 32 kDa |
| Gene ID (Human) | 6129 |
| Human Swiss-Prot No. | P18124 |
| Cellular Localization | Nucleus, nucleolus, cytoplasm, cytosol, ribosome, focal adhesion, membrane, cytosolic large ribosomal subunit, intracellular ribonucleoprotein complex, extracellular exosome |
| Species Reactivity | Human; Mouse; Rat; Cat |
Background
Ribosomes are organelles responsible for catalyzing protein synthesis, consisting of a small 40S subunit and a large 60S subunit. These subunits are composed of four RNA species and approximately 80 distinct proteins. The RPL7 gene encodes a ribosomal protein that is part of the 60S subunit and belongs to the L30P family.
The protein contains an N-terminal basic region-leucine zipper (BZIP)-like domain and an RNP consensus submotif (RNP2). In vitro, the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, showing preference for 28S rRNA and mRNA.
RPL7 can inhibit cell-free translation of mRNAs, suggesting a regulatory role in the translation process. It is located primarily in the cytoplasm and has been identified as an autoantigen in systemic autoimmune diseases such as systemic lupus erythematosus.
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