ELK Biotechnology PKAα/β/γ cat rabbit pAb

제품명

PKAα/β/γ cat rabbit pAb

공급업체

ELK Biotechnology

대체 명칭

PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACG; cAMP-dependent protein kinase catalytic subunit gamma; PKA C-gamma

적용 분야

WB, IHC, IF, ELISA

권장 희석 배수

• Western Blot: 1/500 - 1/2000
• Immunohistochemistry: 1/100 - 1/300
• Immunofluorescence: 1/200 - 1/1000
• ELISA: 1/20000
  (Other applications not yet tested)

면역원

Synthesized peptide derived from human PKA alpha/beta CAT (AA range: 166–215)

보관 조건

-20°C / 1 year

항체 정보

세포 내 위치

Cytoplasm, Cell membrane, Nucleus, Mitochondrion, Membrane (Lipid-anchor).
Translocates into the nucleus as monomeric catalytic subunit.
Inactive holoenzyme found in cytoplasm.
Distributed throughout cytoplasm in meiotically incompetent oocytes; associates with mitochondria as meiotic competence is acquired.
Aggregates around germinal vesicles (GV) at immature GV stage oocytes.
Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (PubMed:21085490).
Isoform 2: Cell projection (cilium, flagellum), Cytoplasmic vesicle (secretory vesicle, acrosome).
Expressed in midpiece region of sperm flagellum (PubMed:10906071).
Colocalizes with MROH2B and TCP11 on acrosome and tail regions in round spermatids and spermatozoa.

배경 정보

This gene encodes one of the catalytic subunits of protein kinase A (PKA), which exists as a tetrameric holoenzyme with two regulatory and two catalytic subunits in its inactive form.
Upon cAMP binding, the holoenzyme dissociates into regulatory dimers and free catalytic monomers.
Four regulatory and three catalytic subunits are identified in humans.
PKA-mediated phosphorylation regulates differentiation, proliferation, and apoptosis.
Constitutive activation due to somatic mutations or gene duplication is linked to adrenal cortex hyperplasia and corticotropin-independent Cushing’s syndrome.

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