ELK Biotechnology PDHK1 rabbit pAb
PDHK1 rabbit pAb는 인간, 마우스, 랫트에 반응하는 다클론 항체로 IF, WB, IHC, ELISA에 사용됩니다. 합성 펩타이드 유래 항원으로 제작되었으며, 세포 내막 및 핵에 위치한 PDHK1 단백질 검출에 적합합니다. -20°C에서 1년 보관 가능합니다.
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제품명
PDHK1 rabbit pAb
제품 개요
PDHK1 rabbit pAb는 인간 PDK1 유래 합성 펩타이드(AA 210–259)를 면역원으로 제작된 토끼 다클론 항체입니다. 다양한 응용 분야에서 PDHK1 단백질의 검출에 활용됩니다.
제품 정보
| 항목 | 내용 |
|---|---|
| Alternative Names | PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1 |
| Applications | IF; WB; IHC; ELISA |
| Recommended Dilutions | IF: 1:50–200 WB: 1:500–1:2000 IHC: 1:100–1:300 ELISA: 1:10000 Not yet tested in other applications |
| Immunogen | Synthesized peptide derived from human PDK1 (AA range: 210–259) |
| Host | Rabbit |
| Storage | -20°C, 1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band | 60 kDa |
| Gene ID (Human) | 5170 |
| Human Swiss-Prot No. | O15530 |
| Species Reactivity | Human, Mouse, Rat |
세포 내 위치 (Cellular Localization)
Cytoplasm, Nucleus, Cell membrane (Peripheral membrane protein), Cell junction (focal adhesion).
Tyrosine phosphorylation occurs mainly at the cell membrane. Translocates to the membrane upon insulin stimulation through interaction with GRB14 and INSR.
SRC and HSP90 promote membrane localization. Nuclear localization depends on association with PTPN6 and phosphorylation at Ser-396.
Restricted to nucleus in neuronal cells, cytoplasmic in non-neuronal cells. Ser-241 phosphorylated form localized perinuclearly in neuronal cells; in both nucleus and cytoplasm in non-neuronal cells. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1.
배경 (Background)
- Catalytic activity: ATP + a protein = ADP + a phosphoprotein
- Function: Phosphorylates and activates PKB/AKT, PKA, PKC-zeta, RPS6KA1, RPS6KB1. Plays a general role in signaling and development.
- PTM: Phosphorylated on tyrosine and serine/threonine residues. Autophosphorylation on Ser-241 required for full activity.
- Similarity: Member of protein kinase superfamily, AGC Ser/Thr protein kinase family, PDK1 subfamily. Contains 1 PH domain and 1 kinase domain.
- Subcellular location: Membrane-associated after stimulation; tyrosine phosphorylation mainly at plasma membrane.
- Subunit: Interacts with TUSC4.
- Tissue specificity: Expressed ubiquitously.
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