Thermo Fisher Scientific TNFA Monoclonal Antibody (OTI1C1), TrueMAB
인간 TNFα를 인식하는 단클론 항체로 Western blot, ICC/IF, Flow Cytometry에 사용 가능. 마우스 IgG2a 아이소타입, 비결합형 항체. HEK293T 세포에서 발현된 TNF 전장 단백질을 면역원으로 사용. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:4,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100 | – |
| Flow Cytometry (Flow) | Assay-dependent | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C1 |
| Immunogen | Full length human recombinant TNF protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It exists as multimers but appears as a single 17 kDa band on SDS-PAGE under non-reducing conditions. Knockout studies in mice suggest neuroprotective roles, and TNF alpha induces tumor necrosis in tumor-bearing mice. It plays a key role in immune responses to various infections and is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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