Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 680

Applications

Tested Dilution

Western Blot (WB)

1:1,000-1:20,000

Immunohistochemistry (IHC)

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

Flow Cytometry (Flow)

1:50 - 1:200

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Rabbit IgG-heavy and light chain

Conjugate

DyLight™ 680 DyLight™ 680 DyLight™ 680

View additional formats

• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/AP/host/Donkey/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Biotin/host/Donkey/)
• [DyLight 350](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 350/host/Donkey/)
• [DyLight 488](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 488/host/Donkey/)
• [DyLight 550](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 550/host/Donkey/)
• [DyLight 594](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 594/host/Donkey/)
• [DyLight 650](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 650/host/Donkey/)
• [DyLight 755](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 755/host/Donkey/)
• [DyLight 800](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/DyLight 800/host/Donkey/)
• [FITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/FITC/host/Donkey/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/HRP/host/Donkey/)
• [Rhodamine](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/Rhodamine/host/Donkey/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L) Cross-Adsorbed/conjugates/TRITC/host/Donkey/)
• Request custom conjugation

Excitation/Emission Max

676/705 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2556622

Target

IgG

Cross Adsorption

Against bovine, chicken, goat, human, mouse, pig and rat

Antibody Form

Whole Antibody

Product Specific Information

This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, goat, human, mouse, pig and rat.

For Western blot applications, 5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.