Thermo Fisher Scientific Phospho-Arhgap22 (Ser397) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot and freeze at -20°C or below for extended storage.
• Avoid repeated freeze/thaw cycles.
• Centrifuge if product is not clear after standing.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only immediately prior to use.

Specificity:
Anti-ARHGAP22 pS397 is specific for phosphorylated ARHGAP22 at Serine 397.
It recognizes the S22→A mutation but not the S397→A mutation.
BLAST analysis suggests cross-reactivity with Mouse, Rat, and Human (100% sequence homology).
Cross-reactivity with other sources has not been determined.

Target Information

GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in down-regulation of their active form.
ARHGAP22 (Rho GTPase activating protein 22), also known as RHOGAP2, is a 698 amino acid cytoplasmic protein containing one Rho-GAP and one PH domain.
It functions as a GTPase-activating protein for Rac1, converting it to an inactive GDP-bound state, and may be involved in transcription regulation through interaction with ZNF161 (VEZF1).
Multiple isoforms of ARHGAP22 exist due to alternative splicing.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.