Thermo Fisher Scientific Phospho-PLK1 (Thr210) Recombinant Rabbit Monoclonal Antibody (ARC1570)
인간 PLK1 Thr210 인산화 부위를 인식하는 재조합 토끼 단일클론 항체입니다. Western blot 및 ELISA에 적합하며, 세포 분열 및 암 연구에 활용됩니다. 고순도 Affinity Chromatography로 정제되어 안정적인 결과를 제공합니다.
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Thermo Fisher Scientific Phospho-PLK1 (Thr210) Recombinant Rabbit Monoclonal Antibody (ARC1570)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1570 |
| Immunogen | A synthetic phosphorylated peptide around T210 of human PLK1 (P53350) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.98 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849766 |
Product Specific Information
Immunogen sequence: KKTLC
Target Information
PLK1 is a serine/threonine kinase involved in cell mitosis and serves as a marker of cell proliferation. It plays a critical role in carcinogenesis. Polo-like kinase proteins act by binding and phosphorylating proteins that are already phosphorylated on specific motifs recognized by POLO box domains.
PLK1 phosphorylates various substrates including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, and WEE1.
It plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1, and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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