Invitrogen™

Platinum™ Tfi DNA Polymerase

Platinum™ Tfi DNA Polymerase combines Platinum™ automated hot-start technology with a non-Taq, surfactant-free PCR enzyme derived from Thermus filiformis. PCR자세히 알아보기

Platinum™ Tfi DNA Polymerase combines Platinum™ automated hot-start technology with a non-Taq, surfactant-free PCR enzyme derived from Thermus filiformis. PCR performance is comparable to Platinum™ Taq DNA polymerase in yield, specificity, fidelity, and robustness for targets less than 1 kb in length. In addition, Platinum™ Tfi is available in a 5Exo (-) form for PCR applications that do not require the polymerase to have 5exonuclease activity. With Platinum™ Tfi, you`ll:–Improve PCR sensitivity and yield with an automated hot start

–Generate more available enzyme during the enzyme activation step for improved PCR robustness
–Amplify more DNA with less enzyme per reaction

Available for OEM: Unlike Platinum™ Taq DNA Polymerase, a customized version of Platinum™ Tfi DNA Polymerase can be made available for resale as a part of a PCR kit for basic research, diagnostics, or other applied fields. Please contact an Invitrogen representative for more information.

Applications: Amplification of DNA from complex genomic, viral, and plasmid templates.

Unit definition: One unit of Platinum™ Tfi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into DNA in 30 minutes at 74°C.

Contents: Platinum™ Tfi DNA Polymerase is supplied with a magnesium free 5X Platinum™ Tfi Reaction Buffer and a separate vial 50 mM MgCl2 .

Contents and Storage:
Platinum™ Tfi DNA Polymerase is supplied with 5X Platinum™ Tfi Reaction Buffer and 50 mM Magnesium Chloride. Store at -20°C (non-frost-free).

사양

GC-Rich PCR PerformanceLow

중합효소Tfi DNA Polymerase

반응 속도Standard

엑소뉴클레아제 활성5- 3

제품라인Platinum™

수량500 unit

배송 조건Dry Ice

Fidelity (Taq 대비)1 X

핫 스타트Built-In Hot Start

오버행3`-A

반응 형식Separate Components

크기(최종 제품)1 kb or less

Unit SizeEach