Gibco™

RPMI 1640 Medium, no glucose

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial자세히 알아보기

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With	Without
• L-glutamine	• Glucose
• Phenol Red	• HEPES

The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagles Minimal Essential Medium or Dulbeccos Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

사양

세포주HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas

세포 유형Leukemic Cells

분류Animal Origin-free

농도1 X

형태Liquid

제품 유형RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)

Serum LevelStandard Serum Supplementation

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

첨가제 포함Glutamine, Phenol Red

첨가제 없음No Glucose, No HEPES, No Sodium Pyruvate

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량500 mL

배송 조건Room Temperature

Unit SizeEach