Thermo Fisher Scientific C-Myc Polyclonal Antibody, Biotin

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 1:50–1:250

Product Specifications

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a key role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. Phosphorylation at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is associated with cell cycle regulation.
c-Myc is essential for vasculogenesis and angiogenesis in tumor development. Deregulated expression induces apoptosis in different cell types. Antibodies against c-Myc recognize overexpressed proteins containing Myc epitope tags. Amplification of c-Myc is observed in lung, breast, and colon carcinomas, while N-Myc amplification occurs in neuroblastomas. Translocation of the c-Myc locus is linked to Burkitt’s lymphoma and other B-cell proliferative disorders. Aberrant expression of c-Myc is found across numerous tumor types including colorectal, gastric, hepatic, ovarian, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.