Thermo Fisher Scientific PLK1 Monoclonal Antibody (OTI1A2), TrueMAB
인간 PLK1 단백질을 인식하는 마우스 단클론 항체로, 세포 분열 및 암 연구용으로 적합합니다. WB, IHC, Flow, IP 등 다양한 응용 가능. 고순도 액상형 제품으로 안정적 저장 및 높은 특이성 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A2 |
| Immunogen | Full length human recombinant protein of human PLK1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.9 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Target Information
PLK1 is a serine/threonine kinase involved in cell mitosis and is regarded as a marker of cell proliferation. It plays a critical role in carcinogenesis. Polo-like kinase proteins act by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains.
PLK1 phosphorylates multiple substrates including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1, and WEE1.
It plays a key role in centrosome function and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1, and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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