Thermo Fisher Scientific Phospho-eIF4EBP1 (Ser65) Polyclonal Antibody
Phospho-eIF4EBP1 (Ser65) 폴리클로날 항체로, 인간, 생쥐, 랫트 시료에 반응합니다. Western blot, IHC, ICC, ELISA 등 다양한 응용에 사용 가능하며, Protein A로 정제된 액상 형태입니다. 번역 억제 단백질의 인산화 연구에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from mouse 4EBP1 around the phosphorylation site of Ser65 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01 M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction with eIF4E inhibits complex assembly and represses translation. The protein is phosphorylated in response to various signals, including UV irradiation and insulin signaling, leading to dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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