Thermo Fisher Scientific Cytokeratin 8 Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 사용 가능한 Cytokeratin 8 폴리클로날 항체. Human 및 Mouse 반응성. 항원 친화 크로마토그래피로 정제된 액상 포맷. 세포 구조 유지 및 신호전달 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 227–483 of Cytokeratin 8 (Uniprot ID: P05787) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.66 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552114 |
Product Specific Information
- Recommended positive controls: HeLa, HepG2, JC
- Predicted reactivity: Mouse (86%), Rat (85%), Dog (88%), Pig (82%), Rhesus Monkey (99%), Bovine (84%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cytokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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