Thermo Scientific™

Pierce™ Glutathione Superflow Agarose

Thermo Scientific Pierce Glutathione Superflow Agarose consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose that자세히 알아보기

Thermo Scientific Pierce Glutathione Superflow Agarose consists of glutathione that has been immobilized to a crosslinked 6% beaded agarose that is designed for high-purity, large-scale purification of GST-tagged proteins.

Features of Glutathione Superflow Agarose:

• High dynamic binding capacity – binds 10 mg of pure GST per mL of resin at a linear flow rate of 150 cm/hour; can bind greater than 20 mg/mL at 30 cm/hour flow rate
• High purity – provides greater than 90% purity when used to purify overexpressed GST or GST-fusion proteins from lysates
• Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200 cm/hour
• Compatible – maintains function after exposure to a wide variety of chemicals and pH values
• Cost effective – resin is stable through multiple cycles of cleaning and reuse

Pierce Glutathione Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates required for medium- to large-scale FPLC purifications. The resin holds up well to a variety of chemical and pH values, and it is compatible with common clean-in-place procedures. The specific binding of glutathione to the GST-tag results in high purity and high yield in a single step purification process.

Applications:
• Medium- to large-scale FPLC purification of GST-tagged proteins

Affinity chromatography is often used as a quick and easy approach for the purification of recombinant proteins. One of the more common recombinant protein purification methods involves expressing a target protein as a fusion with glutathione S-transferase (GST) and then purifying it via the strong and highly specific interaction of GST to glutathione which has been immobilized on a beaded support.

Pierce Glutathione Superflow Agarose enables high-purity purification of GST fusion proteins using medium to high flow rates. Glutathione has been immobilized by its central sulfhydryl group via a 12-atom spacer arm to a highly crosslinked 6% beaded Superflow agarose. To demonstrate performance, GST was overexpressed and purified on a 48L scale. Biomass (170 g) containing overexpressed GST was lysed with 1.7 L of lysis buffer. The lysate was clarified using centrifugation and split into two fractions. The target protein was then purified using Pierce Glutathione Superflow Agarose and another leading supplier`s resin in equivalent 200 mL packed bead columns. The total yield, recovery, and purity (>95%) were nearly identical for both resins.

More Product Data
• Performance characterization of Pierce Glutathione Superflow Agarose

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Pierce™ Glutathione Agarose

사양

용량(미터법)10 mg Pure GST Binding per mL Resin at 150 cm/hour Flow Rate, 20 mg per mL at 30 cm/hour Flow Rate

제품라인Pierce™

수량10 mL

타겟GST-Tagged Protein

유형Agarose

정지상Glutathione

형식Bottle

컬럼 유형Affinity, Agarose Resin

Unit SizeEach