Thermo Fisher Scientific GTF2E1 Monoclonal Antibody (1E12)
GTF2E1 단백질을 인식하는 Mouse IgM 단일클론 항체로, Western blot 및 ELISA에 적합합니다. 비접합(Unconjugated) 형태의 액상 제품이며, 인간 단백질에 반응합니다. -20°C에서 보관하며 반복 동결·해동은 피해야 합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1E12 |
| Immunogen | GTF2E1 (NP_005504, 41 a.a. approximately 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Storage Buffer | Ascites |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
SCVKEEDMLE LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRHN YYFINYRTLV NVVKYKLDHM RRRIETDERD STNRASFKCP VCSSTFTDLE
Target Information
The general transcription factor TFIIE plays an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta), and joins the preinitiation complex after RNA polymerase II and TFIIF.
TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay.
Its sequence contains several structural motifs (leucine repeat, zinc finger, helix-turn-helix) and sequence similarities to bacterial sigma factors, suggesting direct involvement in transcription initiation regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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