
Thermo Fisher Scientific Cytokeratin 4 Polyclonal Antibody
Thermo Fisher Scientific의 Cytokeratin 4 Polyclonal Antibody는 인간 시료에 반응하는 토끼 유래 다클론 항체로, Western blot, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 액상 형태로 제공됩니다. 세포 분화 및 상피 조직 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to a region within amino acids 317–505 of Human Cytokeratin 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_2546939 |
Product Specific Information
- Recommended positive control: HeLa
- Predicted reactivity: Dog (88%)
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Cytokeratins are a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between approximately 40 kDa and 68 kDa, with isoelectric points between pH 4.9–7.8.
Human cytokeratins are numbered 1–20. Expression patterns vary depending on epithelial type and differentiation stage, making cytokeratin profiling valuable for distinguishing epithelial malignancies.
Cytokeratin antibodies assist in tumor differential diagnosis using immunohistochemistry and are also useful in cytopathology and flow cytometric assays.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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