Thermo Fisher Scientific PKM2 Polyclonal Antibody
PKM2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, IP 등 다양한 응용에 적합합니다. 고순도 항원 친화 크로마토그래피 정제, 인간·마우스·랫트 반응성, 안정적인 액상 형태로 연구용에 최적화되어 있습니다.
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Thermo Fisher Scientific PKM2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 243–531 of Human Pyruvate Kinase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.13 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546176 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat brain
- Predicted reactivity: Mouse (97%), Rat (88%), Xenopus laevis (90%), Rabbit (97%), Chicken (83%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PKM2 (Pyruvate Kinase M2) catalyzes the conversion of phosphoenolpyruvate to pyruvate and ATP. It interacts with thyroid hormone and may mediate metabolic effects induced by thyroid hormones. PKM2 also binds Opa protein, involved in gonococcal adherence and invasion of human cells, suggesting a role in bacterial pathogenesis.
Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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