Thermo Fisher Scientific MultiShot StripWell Mach1 T1 Phage-Resistant Chemically Competent E. coli

Invitrogen™ MultiShot™ StripWell Mach1™ T1 Phage-Resistant Chemically Competent E. coli

MultiShot StripWell Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells packaged in a rack containing 12 strips of 8 tubes for increased productivity of bacterial transformations.
The Mach1 T1R strain is derived from a non-K-12, wild-type W E. coli strain (ATCC #9637) selected for rapid growth and engineered to possess useful cloning strain features.

T1/T5 Phage Resistance

All T1R strains have the tonA (also known as fhuA) deletion conferring resistance to T1 and T5 phage. This resistant genotype protects valuable clones and libraries from phage infection, which is critical for genome and sequencing centers.

Accelerate Cloning Workflow

Mach1 T1R competent cells double in approximately 50 minutes (vs. 70 minutes for typical strains). Colonies are visible within 8 hours of plating (ampicillin selection), allowing same-day colony picking. Plasmid DNA can be prepared 4 hours after inoculating a single overnight-grown colony.
Transformation efficiencies in the MultiShot StripWell format exceed >1 × 10⁸ cfu/μg with plasmid DNA.

Genetic Features

The Mach1 T1R strain includes:

• tonA mutation for T1/T5 phage resistance
• lacZΔM15 for blue-white color screening
• recA1398 mutation for reduced homologous recombination
• endA1 mutation for increased plasmid yield and quality
• hsdR deletion for efficient transformation of unmethylated DNA

Genotype:
F– φ80_lacZΔM15 ΔlacX74 hsdR(rK–, mK+) ΔrecA1398 endA1 tonA

MultiShot StripWell Format

Each StripWell of eight connected tubes allows all transformation steps—from DNA addition to plating—in the same tube, minimizing contamination and saving time.
The strips can be cut for single-tube transformations, reducing cell waste and freeze-thaw cycles.

Benefits of MultiShot StripWell Format:

• Increased productivity for high-throughput experiments
• Maximal flexibility (use 1–96 tubes)
• Convenient workflow—add DNA, heat shock, and recover in the same tube

Related Products

Various E. coli strains and formats of chemically and electrocompetent cells are available to meet diverse transformation needs.
For high-throughput applications, explore our MultiShot competent cell collection or contact us for custom formatting options.
Bacterial growth media are also available in multiple formats.

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