Invitrogen™

MultiShot™ StripWell Mach1™ T1 Phage-Resistant Chemically Competent E. coli

MultiShot StripWell Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells packaged in a rack containing자세히 알아보기

MultiShot StripWell Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells packaged in a rack containing 12 strips of 8 tubes for increased productivity of bacterial transformations. The Mach1 T1R strain is derived from a non-K-12, wild-type W E. coli strain (ATCC #9637) that was selected for its rapid growth rate, and genetically manipulated to possess the most useful cloning strain features.

All of our T1R strains have the ton_A (also known as _fhu_A) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse _E. coli hosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

Accelerate cloning workflow using Mach1 T1R strain
MultiShot StripWell Mach1 T1R competent E. coli cells are fast growing chemically competent cells with a doubling time of approximately 50 minutes, with other popular strains averaging 70 minutes. Mach1-T1R colonies are clearly visible within 8 hours of plating the transformation mix (ampicillin selection), enabling you to plate and pick colonies the same day. You can also prepare plasmid DNA 4 hours after inoculating a single, overnight-grown colony in the selective media of choice (with any antibiotic). The Mach1 T1R strain is useful for many cloning applications and provides transformation efficiencies in the MultiShot StripWell format of >1 x 108 cfu/μg with plasmid DNA.

The Mach1 T1R strain was engineered to carry the _ton_A mutation that confers resistance to T1 and T5 phage infection. The _ton_A genotype offers added security to valuable clones and libraries. The Mach1 T1R strain has the _lac_ZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries the _rec_A1398 mutation that helps reduce the rate of recombination while propagating plasmid DNA, the _end_A1 mutation that increases plasmid DNA yield and quantity, and _hsd_R restriction system deletion to allow genomic DNA cloning.

MultiShot StripWell Mach1 T1R Competent Cells offer:
• Transformation efficiencies in StripWell format of >1 x 108 cfu/μg plasmid DNA
• _ton_A (_fhu_A) genotype to confer resistance to T1 and T5 phage
• _lac_Z∆M15 for blue-white color screening of recombinants
• _hsd_R mutation for efficient transformation of unmethylated DNA from PCR applications
• _rec_A1398 mutation for reduced occurrence of homologous recombination in cloned DNA
• _end_A1 mutation for increased plasmid yield and quality

MultiShot StripWell flexible format
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and reducing chance of contamination. After addition of DNA, MultiShot StripWell competent cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube.

The StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots of cells and avoiding freeze-thaws that can result in reduced transformation efficiency.

Benifits of the MultiShot StripWell format include:
• Increased productivity—suitable for high throughput experimentation
• Maximal flexibility—transform as few as 1 or as many as 96 tubes from the StripWell rack
• Familiar convenience—add DNA directly to cells, heat shock, and recover in the same tube

Genotype
F– φ80_lac_ZΔM15 Δ_lac_X74 _hsd_R(rK–, mK+) Δ_rec_A1398 _end_A1 _ton_A

Find the strain and format that fits your needs
A variety of E. coli strains and formats of chemically competent and electrocompetent cells are available to meet transformation needs.
For high-throughput transformation choose from our collection of MultiShot comp cells or email us for custom formatting options.
We offer bacterial growth media in various formats.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝No

형질전환 효율 수준High Efficiency (>1 x 109 cfu/μg)

항생제 내성 박테리아No

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성High

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)Yes

종E. coli (wild-type W)

형식8-well Strips

제품라인MultiShot™

수량1 x 96 tube rack (50 μl/tube)

Unit SizeEach