Thermo Fisher Scientific Occludin Polyclonal Antibody
Occludin 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC 등 다양한 응용에 사용 가능. Human, Mouse, Rat 등에서 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS 완충액에 0.1% sodium azide 포함, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 46 publications |
| Immunohistochemistry (IHC) | – | View 27 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 6 µg/mL | View 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | View 15 publications |
| Immunoprecipitation (IP) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Chicken, Dog, Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from the C-terminal region of the human occludin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533468 |
Product Specific Information
This antibody specifically recognizes the human occludin protein.
In western blot analysis, it detects a target band at approximately 65 kDa.
Reactivity has been confirmed with human Caco-2 and HT29, dog MDCK, mouse TCMK, and rat KNRK cell lysates, as well as mouse kidney, liver, and rat liver homogenates.
It is also validated in Caco-2 cells by immunofluorescence and paraffin-embedded human small intestine tissue by immunohistochemistry.
- For immunofluorescence using Caco-2 cells, fixation with cold ethanol is recommended.
- For immunohistochemistry in paraffin-embedded tissues, enzyme digestion with pepsin is required prior to staining.
Target Information
Occludin is a 65 kDa protein that can exist in multiple phosphorylated forms up to approximately 82 kDa.
It plays a key role in regulating tight junction localization and function.
Polyunsaturated fatty acids up-regulate occludin expression, enhancing transendothelial resistance and reducing permeability to large molecules.
Occludin expression varies by tissue: it is highly expressed in brain tissue and lower in non-neural tissues.
Under-expression of occludin contributes to increased permeability of tumor endothelial junctions, associated with brain tumor edema.
The structural features of occludin are highly conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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