Takara S1 Nuclease

More Information

Applications

• Nuclease protection assays
• Double-stranded DNA end blunting
• RNA transcript mapping
• cDNA hairpin removal

Source

Aspergillus oryzae

Storage

–20°C

Buffer

Supplied with 10X Reaction Buffer: 300 mM sodium acetate (pH 4.6), 2.8 M NaCl and 10 mM ZnSO4

Unit definition

One unit is defined as the amount of enzyme required to convert 1 µg of heat-denatured calf thymus DNA into an acid-soluble product in 1 minute at 37°C and pH 4.6.

Concentration

100–200 U/µl

Product citations

Ando, T. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae. Biochim. Biophys. Acta 114, 158–68 (1966).

Berk, A. J. & Sharp, P. A. Spliced early mRNAs of simian virus 40. Proc. Natl. Acad. Sci. U. S. A. 75, 1274–8 (1978).

Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor laboratory press. New York (1989). doi:574.873224 1/​1989

Wiegand, R. C., Godson, G. N. & Radding, C. M. Specificity of the S1 nuclease from Aspergillus oryzae. J. Biol. Chem. 250, 8848–55 (1975).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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