Takara S1 Nuclease

Takara S1 Nuclease

Applications

• Nuclease protection assays
• Double-stranded DNA end blunting
• RNA transcript mapping
• cDNA hairpin removal

Source

Aspergillus oryzae

Storage

–20°C

Buffer

Supplied with 10X Reaction Buffer:
300 mM sodium acetate (pH 4.6), 2.8 M NaCl, and 10 mM ZnSO₄

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 µg of heat-denatured calf thymus DNA into an acid-soluble product in 1 minute at 37°C and pH 4.6.

Concentration

100–200 U/µl

Product Citations

• Ando, T. Biochim. Biophys. Acta 114, 158–68 (1966).
• Berk, A. J. & Sharp, P. A. Proc. Natl. Acad. Sci. U.S.A. 75, 1274–8 (1978).
• Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York (1989).
• Wiegand, R. C., Godson, G. N. & Radding, C. M. J. Biol. Chem. 250, 8848–55 (1975).

Additional Product Information

Please refer to the product’s Certificate of Analysis for details on storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.

Takara Bio USA, Inc.
United States/Canada: +1.800.662.2566
Asia Pacific: +1.650.919.7300
Europe: +33.(0)1.3904.6880
Japan: +81.(0)77.565.6999

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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