Thermo Fisher Scientific Caspase 3 Monoclonal Antibody (MAR-1), eBioscience

Thermo Fisher Scientific Caspase 3 Monoclonal Antibody (MAR-1), eBioscience

Applications

• Western Blot (WB): Assay-dependent
• ELISA: Assay-dependent

Product Specifications

Product Specific Information

Caspases are present in all cells, and recruitment of these proteases to oligomerized receptors leads to activation accompanied by autoproteolytic cleavage. Active caspases can proteolyze additional caspases, generating a cascade that cleaves proteins critical for cell survival. The final outcome of this signaling pathway is controlled cell death, termed apoptosis.

Caspase-3 cleaves substrates at the carboxyl terminus of aspartate residues. Active caspase-3 consists of two identical large (~20 kDa) and two identical small (~10 kDa) subunits derived from precursor polypeptides. Both subunits contribute to substrate binding and catalysis, with the active site cysteine near the C-terminus of the large subunit. Caspase-3, together with caspases 8 and 9, plays a pivotal role in apoptotic pathways, amplifying initiation signals into full apoptotic disassembly.

Applications Tested: ELISA, Western Blotting.

Target Information

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution phase of apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing to form active enzymes composed of large and small subunits. Caspase-3 cleaves and activates caspases 6, 7, and 9, and is itself processed by caspases 8, 9, and 10. It is a predominant caspase involved in cleavage of amyloid-beta precursor protein, associated with neuronal death in Alzheimer’s disease. Alternative splicing results in two transcript variants encoding the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.